I can extract side effects

"How much does it cost to clone your pet?"

"What's it like to clone a dead pet and regenerate it?"

"Dead cats, clones come back home."

"The grandma in Haishi spent 180,000 yuan to clone pets. His grandson said that grandma's pet dog will die soon due to old age and sickness. In order to let the pet dog accompany her in another form, he and grandma finally decided to spend 180,000 yuan after consulting. 180,000 yuan to buy pet cloning services.”

When Li Chengmin saw the news about cloning pets on the Internet, he was immediately hit.

All along, his wish is to hope that his pet can always be by his side.

Since he was a child, his parents were busy with work and had little time to take care of him. As an only child, he was very lonely since he was a child.

This situation intensified when he was in middle school, due to the pressure of entering a higher school, and he even had a tendency to be autistic and depressed.

However, a raccoon cat broke into the house and changed all that.

Accompanied by this docile, cute, and extremely humane kitten, his sense of loneliness is no longer so strong, and he has become much more lively and cheerful.

After work, he rented a small apartment by himself and took the cat with him.

After more than ten years of companionship, this long-haired tabby cat named Nut has become a real family member to him.

However, the lifespan of pets is not long compared to that of humans, and they often cannot accompany their owners to the end.

Seeing that Nut is getting old day by day, with more and more gray hair, he is too lazy to walk every day, and he will no longer be with him all the time, but lazily lying on the sofa and sleeping all day long.

He knew that the day Nut left was getting closer and closer, maybe next year, it would leave and return to Meow.

This was undoubtedly an unbearable ending for him. Every time he thought about it, he felt a dull pain in his heart, as if he had lost a close relative.

He also considered adopting another cat, but long-haired cats like Nut are rare, and he insisted on wanting a long-haired cat with a color similar to Nut, and this request has not been met for a long time .

Now, seeing the news of cloning pets, he instantly found a new direction.

Pet cloning seems to be far away from most people's lives, but when it comes to the world's first cloned sheep "Dolly", this case written in biology textbooks will definitely evoke people's memories.

Ten years after Dolly was born, a scientific research team in Bangziguo cloned the first hunting dog. By around 2007, cloned dogs had begun to be commercialized in Bangziguo.

Around 2017, many domestic companies have begun to provide commercial pet cloning services. Cat and dog cloning has become two of the more common types of commercially cloned pets, with prices ranging from 100,000 to more than 400,000.

The price of cloning pets is so high, but it can’t stop pet lovers who are willing to pay. The commercialized pet cloning track continues to attract companies to pour in.

But for Li Chengmin, the price is too expensive.

He has only worked for two years, the salary is not high, the daily expenses in Haishi are very expensive, and he does not have much savings, and it is impossible to get the support of his parents for such a thing. The sky-high cloning fee of nearly 200,000 is really far away beyond his capacity.

After some inquiries, he gave up the idea of ​​commercial cloning with a look of regret.

At this moment, he came into contact with biohacking on the Internet, and his eyes immediately brightened.

Since he can't afford to find a pet cloning agency, he'll do it himself.

Isn't this the spirit of technology popularization of biological DIY?

Many technical materials are readily available on the Internet, and he is not afraid of learning.

To do experiments, you can watch videos and ask experts for advice. Even if you ask professionals to teach, it doesn't cost much.

Reagents, equipment, venues, and the shared laboratory with Longtu are about to start trial operation.

Everything is ready, just need to work hard.

Therefore, he resolutely joined the 'Infinite Gene' biohacking group, consulted Zhang Han and others, and began to study biology and cloning technology from the ground up.

The pet cloning process consists of four steps: extracting cell samples, extracting cells to establish lines, constructing cloned embryos, and implanting into surrogate mothers to develop into new individuals.

In detail, to clone a pet cat, you need to extract a cell sample from the pet, usually a small piece of skin, use the cell sample to establish a somatic cell line, and select a batch of female cats in a suitable estrus state to collect spare oocytes.

Subsequently, the nuclei of mature cat oocytes are removed, and cat somatic cells are transplanted into cat oocytes to construct cloned embryos.

Then the cloned embryos are transplanted into the surrogate mother cat, and the surrogate mother cat can be cloned into a pet cat after pregnancy.

The cloning process for dogs is basically the same as for cats.

After learning, Li Chengmin started to practice. At the same time, he also took videos of all the processes and recorded them for inspection.

Extracting cells is the first step in pet cloning.

He personally extracted the skin and epidermis tissue of old cats at home, and successfully preserved the genes.

Everything is going well, but this simple step costs at least 20,000 yuan in a pet cloning agency.

After the cells are extracted, it is in the laboratory that the sample is used to establish a somatic cell line.

This step was relatively difficult, and he consulted many biohackers, and even found an old classmate who studied biology for advice.

At the same time, he also contacted a caring aunt nearby who adopted stray cats to finance the conditions of sterilization and cat food, in exchange for the qualification to collect oocytes from stray female cats in heat.

Of course, considering that pet cloning may cause harm to the surrogate mother, out of compensatory psychology, he plans to adopt the surrogate mother cat together after the cloning is successful.

Moreover, he only intends to use one surrogate mother for each embryo transfer, trying to minimize the damage to stray female cats.

Pet cloning currently has no legal risks, but it will face moral pressure.

Because pet cloning technology such as cats and dogs has been conquered, but the efficiency of cloning is also related to the quality and proficiency of technicians. If the technology is not mature or proficient, a lot of experiments will be required in the early stage, which will waste a lot of manpower and material costs.

Among them, the input of experimental animals is naturally included.

To successfully clone a cat, the staff need to prepare dozens of cloned embryos and implant them into several surrogate mother cats, and finally a cat can be successfully cloned.

This huge waste of experimental animals has also attracted strong resistance from animal protectionists.

They believe that the commercialization of pet cloning is entirely to satisfy people's selfish desires.

Consumers want an identical pet, but countless laboratory animals will be sacrificed behind the scenes.

These experimental animals will not only be injected with hormones, but also undergo surgery again and again, and endure endless life in cages.

All this is unacceptable to animal protectionists.

Li Chengmin is the kind who is not skilled enough. He can only find other ways to practice as much as possible, such as practicing more on mice first, and learning the most advanced iPS induced pluripotent stem cell technology to increase the success rate.

As a senior cat slave who loves Wu and Wu, he can't sacrifice many experimental animals for his own selfish desires. He can only work hard to hone his skills and minimize the damage to cats.

Fortunately, he works alone and is much more free than the staff of the company, so he can make compromises in this regard.

In this way, it also attracted the puzzlement and doubts of the caring aunt next door.

"Young man, if you want to raise a cat, you can just adopt one. Why bother? There are so many stray cats now that they can't be adopted. What are you going to do about cloning?"

"Is it worth it to spend so much energy?"

Auntie accepted the cat food he sent with a smile on her face, while shaking her head, sending out soul torture.

Li Chengmin was at a loss for words, and it was difficult for him to explain all this clearly to his aunt, so he had to smile wryly.

"Auntie, just take it as an obsession."

"If I don't do it, I will be very sad. After doing it, it will make me happy, and it will be worth it."

The aunt walked away muttering, seemingly incomprehensible, and by the way, also felt that his mind was a little out of order.

About two months later, the cell line was successfully established, and Li Chengmin started cloning.

After overcoming a series of difficulties, he finally constructed three cloned embryos and transplanted them into a surrogate female cat.

The next step is to see if the embryo can survive.

Under the current technical means, the survival rate of embryos is not high, about 10%, and only one out of 10 embryos can survive.

Therefore, some companies that undertake a large number of pet cloning will raise their own surrogate mothers, because the time of pet estrus is different, only if there are enough mothers, can there be enough eggs to meet the order demand every month.

Not only that, when a pet cloning agency signs a contract with a client, it usually sets a delivery order time. In order to avoid accidents affecting the delivery of the order, the agency will find multiple mothers for insemination at the beginning to increase the success rate, which requires sufficient quantity experimental animals.

Li Chengmin only has his own order, and he does not have multiple surrogate cats to use.

After all, the caring aunt next door can't tolerate him experimenting on many female cats again and again.

His fault tolerance rate is not high, so he can only improve his technical level as much as possible to increase the survival rate of embryos as much as possible.

Unfortunately, the god of luck did not favor him at the beginning, and the first embryo transfer was unsuccessful.

After 20 days, the female cat still did not show pregnancy.

This is undoubtedly a huge blow to him.

After some inspections, he restarted the second transplant experiment.

This time, just to be on the safe side, he brought the surrogate civet cat home and took care of it himself.

I make cat food by myself every day, canned snacks are enough, and I also make the female cat very comfortable.

Fortunately, this time it finally worked.

One month later, the female cat's belly was obviously bulging.

Li Chengmin was extremely excited, like an old father waiting for a newborn, waiting for the arrival of the child.

Another month later, the mother cat gave birth to three kittens at home.

Due to different genes, cat clones will have certain appearance differences, which are much more likely than dogs.

Therefore, the three kittens born this time, although they are all long-haired tabby cats, are very beautiful, but the colors are not exactly the same.

Nut has beautiful white gloves, a white belly, and a little more white on his face.

Cihuamao is already a breed with relatively small differences. The clone inherits the white gloves, but there is only one with a white belly, and the color of the face is also slightly different from the original in appearance.

However, Li Chengmin was satisfied. He decided to keep the three kittens, and after the kittens were weaned, he sent the mother cat to be neutered.

I don't know if it's a coincidence or what, the nut walked around the three kittens, sniffed for a long time, and returned to the cat the next day.

Li Chengmin suppressed the sadness in his heart, edited all the videos, and posted them on station B.

"I Clone My Cat - The Growth and Metamorphosis of a Biohacker"

This video was full of gimmicks and instantly attracted a lot of hits, breaking through one million views in just one day.

In the video, Li Chengmin told the ins and outs and showed the whole cloning process.

At the end of the video, with a hint of melancholy in his eyes, he said affectionately.

"Only a week after the successful cloning of the cat, my Nut suddenly returned to the cat. All this made me very happy. Fortunately, I started cloning early, and kept a brand new one before I left."

"I can't believe how sad and regretful I would have been if I hadn't started this sooner and faced its departure."

"Everyone, although cloning technology is difficult, it is not insurmountable. I use my own experience to prove to everyone that as long as you are willing to work hard, you can save the 200,000 yuan and at the same time minimize the damage to the surrogate mother cat. "

"Now this surrogate mother cat has become part of my family. Together with her three children, she has lived a happy feline life."

"Okay, that's the end of the video, if you want to know everything about cloning pets, please follow me. Thank you!"

For the general public, paying 200,000 yuan to clone a pet cat is enough to attract attention and spark public opinion.

What's more, cloning a pet cat by yourself is such a hard-core thing.

Pet cloning, biohacking, these two hot words instantly made the audience's adrenaline soar.

After watching the video, the netizens all gasped in amazement.

"My God, is this the most hardcore up master in the history of station B?"

"My God, is this something I can watch without paying? I'm embarrassed if I don't give you one click and three."

"The senior cat slaves used to be like this. I am ashamed and ashamed. I dare not say that I am a cat slave in the future."

"Master UP, how much did you spend for cloning such a set, can you disclose it?"

"I feel that the cost is not very high. It may be done within 50,000 yuan. The hard part is the energy I put in."

"Master UP, do you accept the business order? I also want to clone the dog at home. I sent you a private message, remember to reply."

"It's biohackers again. This thing is so popular recently, I want to join them."

"The concept of biohacking is really good. It used to be popular abroad, but now it is popular in China."

"If cat slaves can clone their own cats like UP owners, what can my country's biotechnology develop into?"

"This UP master can clone cats now, but I don't even dare to think about cloning anything in the future."

"Professionals said that in fact, there is no technical threshold for pet cloning now, and the commercialization is also very mature. There are many companies in the market that do this business, but the C-end does not make much money. There are only more than 100 cases a year. It was introduced by an acquaintance. The main business is still business-oriented, providing services such as cloning silverback gorillas to zoos, and several companies have completed angel round financing.”

"Now more and more people keep cats and dogs, and the consumer market for pets is also very large, with more than 200 billion yuan. It seems that there will be a market for pet cloning in the future."

"Actually, pet cloning cannot be exactly the same as the original. It can only be regarded as identical twins. Moreover, cloned pets are prone to diseases and have a short lifespan. If you really like keeping cats, you can completely raise another one. There is no difference at all."

"Each pet is unique. Even if it is cloned, its memory and traces of life are difficult to find on the clone. It is equivalent to buying a pet with the same appearance. The significance of cloning is not as much as everyone imagined. big."

"Everyone should act with caution. Of course, it doesn't matter if the UP master clones himself, but we don't have this technology, and it will cost a lot of money. We still have to carefully consider whether it is worth it."

Although pet cloning caused some controversy, all viewers expressed their respect for Li Chengmin's hard-core technology with crazy retweets.

In three days, the number of video views exceeded 10 million, and it continued to grow.

This video also went viral and became a hot search on the Internet. Even TV stations had people interviewing him.

The WeChat marketing account even quickly concocted several small essays, frantically chasing the popularity.

The word biohacking instantly became a hot word on the Internet, just like cyber hackers, it has been widely heard and deeply rooted in the hearts of the people.

Soon, it was discovered that disease-resistant pigs and cloned cats had something in common.

Both Gao Jun and Li Chengmin belonged to the same biohacker group.

The 'Infinite Genes' biohacking group went viral.

It even became popular overseas - the base camp of biohackers.