I can extract side effects

Du Biao came to the door of the laboratory and was about to open the door to enter, but he heard bursts of laughter from inside.

He paused, stopped outside the door, and listened quietly.

"I finally found out why the sows were miscarrying."

"Fuck, I know why."

"What is your reason?"

"I did a genetic analysis and found that some of the eggs' own PERV virus infection genes still exist, so the resulting embryos also contain PERV viruses, and then the embryos disappeared halfway through development."

"Then you didn't remove the nucleus of the oocyte?"

"Yes, maybe when the nucleus was removed, it was not complete enough, leaving some gene fragments, and then infecting the embryo."

"It's easy for you to solve, just clean it up when removing the nucleus. It's miserable for me. My embryo was infected with other viruses from the sow in the womb."

"Hey, do you want to find a cleaner sow again?"

"Isn't it? It's really strenuous to have to start all over again."

"By the way, are we completely wiped out this time?"

"No, Xiao Gao's sow is fine."

"It seems that he is the hope of the whole village. The result of this experiment depends on him."

"Tsk tsk, what did Xiao Gao do to the sow, why should he be fine?"

"Then you'd better ask yourself what you did to the sow, why your sow keeps miscarrying."

"Aren't you too? Hahaha~"

Hearing these words, Du Biao knew what happened.

It turned out that almost all the cloning experiments done by the students failed.

Only one succeeded.

The name Xiao Gao fell into his ears, reminding him instantly of a thin and tall figure.

This student really loves learning and has good hands-on skills. It is not surprising that he can become the last hope of the laboratory.

Seeing that everyone was almost done discussing, Du Biao coughed lightly, opened the door and walked in.

I saw that everyone was gathering together, surrounding a pigsty, and observing the only remaining sow in it.

The sow has gained a lot of weight, the black pattern on her back is no longer obvious, her belly is protruding high, her head is buried in the trough, and she is drinking milk.

"Teacher Du is back."

As soon as he came in, the students heard the movement, looked back and hurriedly said hello.

Seeing all this, Du Biao knew what was going on, and asked with a smile.

"I heard that something went wrong with your experiment."

"If you have any problems, just tell me, let's solve it together."

The students looked at each other for a long time, all eyes fell on Xiao Gao.

Xiao Gao, thin and tall, wearing a pair of black-rimmed glasses, was taken aback when he heard the words, and then hesitated for a while.

"Teacher, do you understand the pregnancy and postpartum care of sows?"

"This..." Du Biao was at a loss for words.

How could he, a professor of cell biology, understand the postpartum care of sows?

What I usually study and study are sliced ​​cells under a microscope.

If it weren't for the new project of xenotransplantation, his research would not have anything to do with pigs.

Xiao Gao saw that the teacher's face became a little unsightly, and hurriedly waved his hands.

"Teacher, it's okay, let's study this by ourselves."

Seeing that the students are so sensible, Du Biao felt a little guilty.

I have rarely stayed in the laboratory in the past six months, and I have not been very dedicated to teaching students.

Right now the students are encountering difficulties in their experiments, but I can't help much, I feel a little ashamed of the students.

He couldn't help but sighed, and said, "Let me be the teacher. I know Professor Wang from the animal medicine next door."

"He is very knowledgeable about the treatment and care of all kinds of common animals. I went to him to learn from him."

Xiao Gao nodded again and again, showing a joyful smile.

"That's great. Now only my embryos are left alive in the lab. I'm wondering what to do to get through the past few months and raise the piglets safely."

Du Biao nodded and smiled, and finally regained a trace of confidence in being a teacher.

"Don't worry, leave everything to the teacher."

*****

"This pig fetus is about to be born, have you checked the genotype?"

"Checked and it was amazing, the surrogate sow had the PERV virus gene, but the fetus was 100% free of PERV."

"It's a miracle of life. The virus in the sow did not infect the fetus this time."

"Most of the minipigs used in the laboratory have been gene-edited, and some of the PERV virus genes may have been removed."

"It makes sense, but it doesn't explain why the virus in my sow can infect the fetus."

"Maybe, this is the power of maternal love in the legend?"

"You might as well call it metaphysics."

"It has nothing to do with metaphysics. The natural world is so magical and full of various abnormalities. Otherwise, there would be no such thing as genetic mutation."

"Minipigs are just fine, no genetic modifications are needed to limit their growth, and their organs are naturally similar to humans and primates."

"I am still a little worried about the condition of the fetus after it is born. There are too many genetic modifications, and God knows what impact it will have."

"Xenotransplantation is like this. It requires large-scale genome engineering. In addition to knocking out the relatively easy PERV, it also needs to insert a large amount of human DNA into the donor pig genome. It is really unavoidable."

"Fortunately, there are Sanqing's gene editing tools, otherwise it would be difficult to knock out large fragments of DNA and complete our experiment."

"Have you ever studied computer programming? I have taken some elective courses in C++ and Java. When knocking out the pig cell genome, I felt like I was writing code."

"Me too, it's all object-oriented programming, encapsulating RNA, and then modifying the DNA code according to the comparison, it feels like the hand of God."

"If we continue to develop like this, we will become code farmers in the new era, but we are coding creatures."

"Thankfully, genetic modification is only done on animals at present, and it has not yet fallen on humans."

"I have a hunch that this kind of thing will happen sooner or later. Do you dare to say that in a certain corner of the world, no one is secretly modifying human genes?"

"The Ethics Committee will not let this happen. Even the genetic modification of animals is restricted, and it cannot be done at will. The modification of the brain and reproductive cells is strictly prohibited."

"Don't think so badly, haven't you noticed? The field of xenotransplantation will develop very well in the future. As long as you have a little ability, you will definitely find a job after graduation."

"Of course, after we finish this experiment, even if we want to enter Sanqing, we can, let alone other biotechnology companies."

"If the big brother didn't propose to do this new project at the beginning, I'm afraid it would be difficult to publish a paper just by following the mentor."

"The tutor was taken away by the elder brother. I also want to hug my thigh tightly and hang out with him in the future."

In the daily chats of the students, time passed quickly, and soon it was time for the sow to give birth.

The miniature pigs used in the experiment are different from ordinary domestic pigs. After improvement, the development time is much faster.

In addition, the embryos are artificially implanted. Compared with domestic pigs with more than a dozen litters, the number is only half, and they can obtain sufficient nutrition for development in the mother.

Therefore, in less than 70 days, 6 piglets were born healthy.

In the first time, it was divided up by the students who liked to be their parents.

However, there are only 6 piglets, but there are 8 students who want to be parents, which is not enough points at all.

In the end, except for Xiao Gao who got a cub without any dispute, the other seven could only draw lots to decide the piggy's ownership.

The fat boy and the ponytail girl unfortunately lost the election.

The two troubled brothers and sisters were so angry that they couldn't help complaining about Xiao Gao.

"Xiao Gao, why didn't you put a few more embryos?"

"Hey, if I had known that your sow would be able to give birth, I should have given you all my embryos."

Xiao Gao also had an aggrieved face, yelling that he was wronged.

"It's really not my fault. I implanted 10 embryos at the beginning, but who knows that 4 died halfway, and they were all absorbed by other embryos."

"The mortality rate of my embryo is quite high. To be honest, I feel incredible that my sow did not have a miscarriage."

"It seems that you are the chosen one."

The fat boy said sourly. After speaking, his nose twitched and his mouth curled up, almost crying.

Xiao Gao couldn't bear it, patted his fat shoulder, and said proudly:

"It's okay, you can help me raise the cubs, I don't mind."

"You..." The fat boy agreed after thinking for a long time, pinching his nose.

The ponytail girl also found another girl, and the two talked for a long time, happily becoming a godmother.

The matter was finally resolved satisfactorily, and the next step was to wait for the piglets to mature and then transplant the organs into the baboons.

During this period, while raising the piglets carefully, everyone was also studying the reasons for the failure of embryonic development.

This is the case with scientific research, failure is not terrible, as long as the experience and lessons are summed up and the next experiment continues to improve, the survival rate of embryos can be greatly improved.

After more than two months, the piglets, which were only the size of a palm at birth, have grown to about 10 kilograms, and they have matured.

The size has also become similar to that of a dog, but it is fatter and looks rounder, white and pink, very cute.

Looking at these 6 healthy and lively piglets, even Huang Qin couldn't help but feel a lot of emotion.

"Worldwide, at least 40 different genes in pigs can currently be edited. We have edited 36, and the knockout virus is already close to this number."

"The piglets with such deep gene editing can grow up healthily. To be honest, even I was surprised."

"Students, you have worked hard. These 6 little pigs were raised by you. Without your efforts, there would be no achievements today."

This is true. After the embryos were implanted in the sow's uterus, he rarely came to the laboratory to give guidance. The specific work of raising the cubs was handed over to each student.

After all, he was too busy to raise pigs.

At present, the students can complete the work so well, which shows that they have put in all their efforts.

Only with such hard work can we reap fruitful results.

"Even animal organ transplantation is very complicated. I have already contacted the animal experiment center and they are ready to start the operation in the afternoon."

"Pick out a pig, do various inspections, and bring it over in a while."

Huang Qin looked at the six genetically modified pigs, nodded with satisfaction, and carefully instructed some precautions.

The students cheered instantly, showing smiles like rain after a long drought.

"Great, after nearly a year of experimentation, I finally waited until this day."

"I'm going to have a transplant soon, and I'm so excited just thinking about it."

"Hey, these are all the babies we have worked so hard to raise, and we are reluctant to pick anyone."

"Then why not draw lots?"

"It can only be this way."

Huang Qin looked at the smiling faces of his classmates, and a smile appeared on his face.

He understands everyone's mood at the moment.

After such a long period of hard work and continuous overcoming difficulties, I finally reached a critical step.

Who can not be excited, who can not look forward to.

Even he is no exception.

Even more than these classmates, I look forward to the results of this operation.

*****

Kunming, Animal Experiment Center.

A group of 10 people put on surgical gowns, masks, hats and gloves, and came to the operating room under the leadership of Dean Liu.

In addition to Huang Qin and eight classmates, Du Biao also rushed over to participate in this historic moment.

Fortunately, the operating room is large enough to accommodate so many people without appearing crowded.

Everyone gathered around the operating table, watching quietly.

On it lay a baboon, baring its teeth, unconscious.

As for the pig that was the donor, it had already been anesthetized and was lying on an operating table not far away.

Two kidney transplant surgeons stood in front of the operating table with serious faces.

They expertly inspected the bodies of baboons and pigs and read out all the parameters.

"Recipient: One female baboon, weighing 20 kg."

"Before receiving xenotransplantation, bilateral native nephrectomy was performed, as well as islet cell resection."

"Serum creatinine index...glycemic index...other renal function parameters..."

"Donor: Selected 36-GE pig, 76 days old, 15 kg, female."

"Insert 21 human immune regulatory and complement inhibitory genes such as CD55, CD46, HO1 and CD47."

"Insert two human anticoagulant genes, TBM and EPCR."

"Insert 4 humanized kidney genes."

"Insert 1 insulin expression gene."

"Delete 2 porcine carbohydrate antigens."

"Deletion of GGTA1, GalNT2, CMAH and other 6 genes that can trigger acute rejection."

"It does not express red blood cell antigens and is a universal donor for any blood type."

"Normal renal function, BUN19, creatinine 1.3, assessed within 30 days prior to kidney donation."

"Porcine endogenous retroviruses and other viruses, showed negative."

"Next, the recipients will be induced with immunosuppression prior to transplantation."

The chief surgeon said in a deep voice, and without any slowness in his hands, he slowly injected the immunosuppressant into the baboon.

Everyone looked serious, and they only nodded slightly when they heard such detailed data.

Even Dean Liu, who was always good at talking, didn't have the heart to talk, and put all his attention on the operation.

In the beeping sound of the monitor.

The belly of the pig was dissected, and the islet cells were transplanted into the baboons.

Both kidneys were taken out one after another and transplanted one after another.

The ureter and bladder were sutured sequentially, followed by the blood vessels.

After the vascular anastomosis is completed, the vascular clips are removed.

After 5-10 seconds, the pale kidneys were quickly perfused and returned to their original blood color.

At this point, the chief surgeon finally breathed a sigh of relief.

"The transplant was a success. Both kidneys reperfused rapidly, with good color and filling."

"Hyperacute rejection has not occurred yet, and the overall performance is good."

"The next step is to see how long it takes to produce urine."